ScRNA-seq

释义 Definition

scRNA-seq 是 single-cell RNA sequencing（单细胞RNA测序） 的缩写：一种在单个细胞水平测量基因表达（RNA转录本）的技术，用于解析细胞类型差异、细胞状态变化与组织异质性。（也常写作 scRNAseq 或 scRNA-Seq。）

发音 Pronunciation (IPA)

/ˌɛs siː ˌɑːr ˌɛn ˌeɪ sɛk/

例句 Examples

We used scRNA-seq to study immune cells.
我们使用单细胞RNA测序来研究免疫细胞。

By combining scRNA-seq with batch correction and clustering, the researchers identified rare cell populations and traced how gene expression changes during disease progression.
研究人员将单细胞RNA测序与批次效应校正和聚类分析结合，识别出了罕见细胞群，并追踪了疾病进展过程中基因表达的变化。

词源 Etymology

scRNA-seq 来自学术写法的缩略：sc = single-cell（单细胞），RNA = ribonucleic acid（核糖核酸），seq = sequencing（测序）。该缩写随着单细胞技术在生物医学研究中的普及而广泛使用。

相关词 Related Words

• Transcriptome
• Sequencing
• Single-Cell
• Gene-Expression
• Clustering
• Cell-Type
• UMI
• Drop-Seq
• Batch-Effect
• Spatial-Transcriptomics

文献与著作中的使用 Notable Works

• Tang, F. et al. (2009). mRNA-Seq whole-transcriptome analysis of a single cell.
• Macosko, E. Z. et al. (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets (Drop-seq).
• Zheng, G. X. Y. et al. (2017). Massively parallel digital transcriptional profiling of single cells.
• Stuart, T. et al. (2019). Comprehensive Integration of Single-Cell Data (Seurat).